Mulyoto obtained his first degree in Animal Husbandry majoring in Animal Reproduction from Jenderal Soedirman University, Indonesia, where he subsequently lectured in Reproductive Physiology. He came to Melbourne in 1993, completing first the Graduate Diploma in Agricultural Science at Melbourne University then Master in Reproductive Sciences at Monash University.
In 1998 he received a scholarship from the Asian Development Bank to continue his doctoral study and he completed his PhD with Dr Jill Shaw and Dr. Orly Lacham-Kaplan on dry storage of biological material. In 2000 he won a Gold Award of Asia-Pacific Young Inventors for his research on sperm dry storage, which was then known as evaporative drying. He completed his PhD in 2002, then worked as a research fellow at Monash Institute of Reproduction and Development.
Mulyoto has been involved with Master in Clinical Embryology since 2001, first as a lab demonstrator for micromanipulation and then as Unit Coordinator in 2003. In 2005 he was appointed as Laboratory Manager and Practical Coordinator for the MClinEmb. Mulyoto helps to set up new IVF clinics in Indonesia and is consultant for four clinics in Indonesia. He also holds a lecturer position in Indonesia where he teaches and conducts workshops in the field of IVF.